Chromatography of proteins on diethylaminoethyl-cellulose in concentrated ammonium sulfate
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چکیده
منابع مشابه
Chromatography of Myoglobin on Diethylaminoethyl Cellulose Columns*
Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the ide...
متن کاملFractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.
Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutin...
متن کاملThyroglobulin fractionation on diethylaminoethyl cellulose columns.
Thyroglobulin may be defined as the iodine-containing protein of the thyroid gland which has a sedimentation coefficient of 4o.w = 19 S, an electrophoretic mobility at pH 8.6 of approximately 5 x 10F5 cm2 volt-l set-1, and a steep salting out curve at about 38% ammonium sulfate saturation at neutral pH (1). Although other iodoproteins, both soluble in neutral salt and attached to cell particles...
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The crystallization by Theorell (8) of one of the hemoglobins of muscle, horse heart myoglobin, has made possible the more accurate study of this interesting protein, and opened the way for the investigation of other members of the series. Previously the question of the existence of a protein in the muscle resembling, but nevertheless distinct from, blood hemoglobin was in doubt. Theorell’s wor...
متن کاملCharacterization of cholesterol-binding globulin by modified zone electrophoresis and O-(diethylaminoethyl) cellulose chromatography.
Normal human serum has been shown' to exhibit cholesterol-binding properties upon immunoelectrophoresis on agar gel. The evidence clearly supports the concept of a specific type of binding of cholesterol by alpha-beta globulin. Accordingly, the name "transcholesterin" was proposed for the reactive component. In order to evaluate the mechanism of in vitro interaction of cholesterol with transcho...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1971
ISSN: 0003-2697
DOI: 10.1016/0003-2697(71)90166-7